Diversity of site-specific microbes from occlusal and proximal lesions in severe early childhood caries (S-ECC)

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J Oral microbiol. 2022 Feb 13;14(1):2037832. doi: 10.1080/20002297.2022.2037832. eCollection 2022.


BACKGROUND: Severe early childhood caries (S-ECC) is a worldwide problem of great concern, typically manifesting on the occlusal and proximal surfaces of affected teeth. Despite the major ecological differences between these two niches, compositional differences, if any, in the microbiota of these lesions are unknown.

METHODS: Samples of deep dentin caries from asymptomatic primary molars of children with S-ECC (n 19) belonging to caries code 5/6 (ICDAS classification) were evaluated. Using two primer pools, we amplified and compared the bacterium 16S rRNA gene sequences of the seven hypervariable regions (V2-V4 and V6-V9) using an NGS-based assay.

RESULTS: Bray-Curtisevaluation indicated that occlusal (OL) lesions had a more homogeneous community than proximal (PL) lesions with significant compositional differences at the species level (p = 0.01; R-0.513). Together, the occlusal and proximal niches housed 263 species, of which 202 (76.8%) species were common to both, while 49 (18.6%) and 12 (4.6%) disparate species were exclusively isolated from the proximal niches and occlusal, respectively. The most common genres in both niches included Streptococcus, Prevotellaand Lactobacillus. S. mutans predominated in PL (p ≤ 0.05), and Atopobium parvulum (p = 0.01) was predominant in LO.

CONCLUSIONS: Distinct differences exist between the caries microbiota of occlusal and proximal caries in S-ECC.

PMID:35173909 | CPM:PMC8843124 | DO I:10.1080/20002297.2022.2037832

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